Studies on ATP-diphosphohydrolase nucleotide-binding sites by intrinsic fluorescence
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چکیده
منابع مشابه
Studies on ATP-diphosphohydrolase nucleotide-binding sites by intrinsic fluorescence.
Potato apyrase, a soluble ATP-diphosphohydrolase, was purified to homogeneity from several clonal varieties of Solanum tuberosum. Depending on the source of the enzyme, differences in kinetic and physicochemical properties have been described, which cannot be explained by the amino acid residues present in the active site. In order to understand the different kinetic behavior of the Pimpernel (...
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血小板は,血 管損傷部位への接着による損傷 部位か らの出血阻止 とい う目的だけでな く,活 性化に伴い種々の生理活性物質 を主 として局所 に放出する.こ の放出反応は種々のメカニズム によって制御されていることは周知の通 りであ る.こ れらの活性物質のうち,ADPは 血小板研 究の歴史の中で最初 に発見された血小板凝集物 質であ り,い くつかの血小板凝集惹起物質の中 では弱い凝集物質 と云 う位置づ けがされている が,ト ロンビンや コラーゲ ンを用いた際 にも ADPは 重要な役割を果たしている.これ までに ADPの 細胞外での分解に関わる酵素が血管内 皮細胞上に存在することが知 られてお り,い く つかのグループが,こ の精製 ・解析 を試みてい た.一 方で,Epstein-Barrウ イルスで刺激され たB細 胞の表面上 に2回 膜貫通型 の細胞抗原 が発現 ...
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The intensity of the tryptophan fluorescence of the alpha subunits of guanine nucleotide-binding regulatory proteins increases when they bind guanosine 5'-O-(3-thio)triphosphate (GTY gamma S). The kinetics of the fluorescence enhancement and of the measured binding of [35S]GTP gamma S are well correlated. The addition of Mg2+ to the nucleotide-bound proteins causes a further, rapid increase in ...
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ژورنال
عنوان ژورنال: Brazilian Journal of Medical and Biological Research
سال: 2000
ISSN: 0100-879X
DOI: 10.1590/s0100-879x2000000700001